15522676233
15522676233
技術文章
更新時間：2022-11-24
點擊次數：1920
PEI又稱為線性化聚乙烯亞胺，是人工合成的含有高密度正電荷的有機大分子陽離子聚合物。PEI與DNA通過正負電荷的相互吸引形成復合物，這些復合物很容易通過內吞作用進入細胞。PEI-DNA復合物能很好地抵御溶酶體的降解作用，有效提高轉染效率。PEI可作用于大至135000個核苷酸的質粒，所以較大的質粒也可以成功轉染。目前市面上PEI轉染試劑有很多種，Polysciences的PEI線性轉染試劑適用于多種真核細胞，用途廣泛。
一、Polysciences線性PEI轉染試劑(PEI 25K)特性：
貨號：23966-1
分子式：(CH2CH2NH)n
分子量：25000
外觀：白色至黃色粉末
熔點：73-75℃
溶解性：溶于：熱水、低pH值的冷水、甲醇和乙醇；
不溶于：苯、YI醚和丙酮。
二、Polysciences聚乙烯亞胺轉染試劑(PEI 25K)的應用：
| 遞送物質 | 轉染細胞類型 | 轉染細胞舉例（包括但不限于） |
| DNA | 貼壁/懸浮細胞 | HEK293 |
| CHO | ||
| HELA | ||
| SF9昆蟲細胞 | ||
| SF21昆蟲細胞等真核細胞系 |
(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9. IF：19.160
試劑：PEI 25K；應用：DNA轉染人胚腎293S細胞。
(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54. IF：10.633
試劑：PEI 25K；應用：質粒DNA轉染小鼠骨髓間充質干細胞（D1）。
(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020. IF：6.630
試劑：PEI 25K；應用：包裝質粒pCMVdelta8.9、包膜質粒VSV-G和PD-1-GFP、PD-L1-GFP或空GFP sFFV轉移質粒轉染HEK293T細胞
(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276. IF：4.395
試劑：PEI 25K；應用：轉染HEK293T細胞。
(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739. IF:4.345
試劑：PEI 25K(23966-1)；應用：質粒轉染HEK293FT細胞。
(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373. IF：3.602
試劑：PEI 25K(23966-1)；應用：用shRNA-PLKO.1質粒轉染HEK293FT細胞。
(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132. IF：3.595
試劑：PEI 25K；應用：質粒DNA轉染懸浮生長的CHO DG44細胞。
(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.
試劑：PEI 25K；應用：用DNA轉染HEK293T細胞。
(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.
試劑：PEI 25K；應用：將編碼fLuc基因的DNA遞送至小鼠。
(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.
試劑：PEI MAX(24765)；應用：用質粒DNA轉染ET801載體—幼倉鼠腎來源細胞。
北京百奧創新科技有限公司提供多款Polysciences轉染試劑，具體信息請咨詢百奧創新客服。(Polysciences PEI 25K 23966-1現貨打折，*！機會有限，不要錯過！)
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